Mosaicism in the inner cell mass of human blastocysts
نویسندگان
چکیده
منابع مشابه
Mosaicism in the inner cell mass of human blastocysts.
Although mosaicism was shown to be a normal feature in cleaving embryos, its consequences for the late preimplantation stages are unknown. We performed blastocyst immunosurgery, followed by fluorescent in-situ hybridization (FISH), to determine the number of cells and degree of mosaicism in the inner cell mass (ICM) of human blastocysts. Of 47 ICM samples analysed, 20 had aneuploid cells, and t...
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The expression of the transcription factor Oct-4 is thought to be one of the decisive factors that maintain totipotency in embryonic and germ cells. In mice, oct-4 is exclusively expressed in germ cells and totipotent cells of the embryo. In humans, Oct-4 is expressed in germ cells, embryonic stem cells and whole embryos at various stages of development. However, there is limited information ab...
متن کاملCell Fate Specification Based on Tristability in the Inner Cell Mass of Mouse Blastocysts.
During development, interactions between transcription factors control the specification of different cell fates. The regulatory networks of genetic interactions often exhibit multiple stable steady states; such multistability provides a common dynamical basis for differentiation. During early murine embryogenesis, cells from the inner cell mass (ICM) can be specified in epiblast (Epi) or primi...
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We have explanted mouse blastocysts into culture dishes and studies the positioning of the inner cell mass (ICM) at the time of attachment in order to establish what effect the location of the inner cell mass has on the subsequent development of embryos in vitro. We show that blastocysts may attach to the substrate with any portion of the trophectoderm. The location of ICM within the attaching ...
متن کاملThe development of trophoblast in vitro from blastocysts containing varying amounts of inner cell mass.
When intact mouse blastocysts are cultured in vitro in medium supplemented with foetal calf serum, trophoblast cells proliferate and undergo giant cell transformation such as occurs in vivo. If the amount of inner cell mass in the blastocyst is decreased by culture with [3H]-thymidine then giant cell transformation occurs normally but proliferation is reduced. In the absence of inner cell mass ...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1998
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/13.11.3151